| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.058537 |
| Chromosome: | chromosome 12 |
| Location: | 8678996 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547950 | (1 of 2) PF08590 - Domain of unknown function (DUF1771) (DUF1771) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCGTTGCAGCGACACGTCCGCTCAGTTGCATTGTTGCCACTAGCAC |
| Internal bar code: | CAGCATAGCTCCTGAGGTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 76 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATCGACTGCAATCGGTC |
| Suggested primer 2: | TATCGCCTCCTTGACCCTCA |