Insertion junction: CLIP2.058539_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.058539
Chromosome:chromosome 17
Location:2754851
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g717850 PHC8 (1 of 1) IPR003882//IPR024616//IPR027417 - Pistil-specific extensin-like protein // Pherophorin // P-loop containing nucleoside triphosphate hydrolase; Pherophorin-chlamydomonas homolog 8 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCCTGATACCGACGCACGCTCCCGGAACACCTTTCGTTCACGCCCTCG
Internal bar code:ATGTAGTATTCTATCATTCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:687
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GTGATGCAAATCTCCGCACC
Suggested primer 2:GCGAGTCTTCTGGGTGACAA