| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.058544 |
| Chromosome: | chromosome 9 |
| Location: | 5208882 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406851 | CDH1 | Activator and specificity factor for anaphase promoting complex; (1 of 1) K03364 - cell division cycle 20-like protein 1, cofactor of APC complex (CDH1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCTCAAATGTGCATGAAACTGTGTATCATGGTTTGCGGCATGGTTGC |
| Internal bar code: | TGCAGTACTCATTCTAAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1709 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGACAAGAGTTGAGTGCG |
| Suggested primer 2: | ACCTCACCTGGCTCAACATG |