| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058550 |
| Chromosome: | chromosome 2 |
| Location: | 8136681 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144000 | IND1 | (1 of 1) PTHR10584//PTHR10584:SF201 - SUGAR KINASE // INDIGOIDINE SYNTHASE A-LIKE PROTEIN; Indigoidine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAGTGCATGGGAATTCAGCATGCACCCAATCAATGTAATGCTGTTTC |
| Internal bar code: | ATTTGTCTGTGTAGTAAATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1449 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTTTCGGAGTTGGACGGC |
| Suggested primer 2: | GGTGGTGGTTCAGAGAAGGG |