| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058570 |
| Chromosome: | chromosome 16 |
| Location: | 7896304 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689871 | TSI1 | (1 of 1) PTHR11946:SF82 - ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; Putative organellar isoleucyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAGGCGGTGGAGGGGCCCATCGCCCACTCACGAATACGCCTTCACC |
| Internal bar code: | ATTGGAGAGTGGTATACCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 76.4706 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTGCGATTCCCTCCTTT |
| Suggested primer 2: | CAACAGCACAAGACAGGCAC |