Insertion junction: CLIP2.058573_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.058573
Chromosome:chromosome 13
Location:3643299
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g588100 CYN19C,ROC2,CYN5,CYN19-3 (1 of 1) K09565 - peptidyl-prolyl isomerase F (cyclophilin D) (PPIF); Cyclophilin 19-3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCCAGCCCATCCCCGCACTCGCGTCCCAACCCCCGTAGGCCAACGCCG
Internal bar code:AGGGGAGAGGTGCCTGTCTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:658
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGTCCCTCTACCCAACGG
Suggested primer 2:ACAGCGCTCGATTCTAGCTC