Insertion junction: CLIP2.058578_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.058578
Chromosome:chromosome 12
Location:8469162
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g549750 FAP4,CK1,CSK1 Flagellar Associated Protein 4; (1 of 2) K02218 - casein kinase 1 (CSNK1, CK1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCCCTCACATTGAACGGTCTAGACTATCTCGCCTAGGACCGGAATGAG
Internal bar code:CTCCTTCGGTTAATGGTCCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2380
LEAP-Seq percent confirming:98.5916
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGCCCCGAATCTCTGTTG
Suggested primer 2:CAGGACTACCATCGCGCTAG