| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.058581 |
| Chromosome: | chromosome 6 |
| Location: | 5618532 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCGCAGTGCCCGCACTGCCCCCAACGCCTCCTCCGCAGCTTTACGCC |
| Internal bar code: | AGACAATGCGATCTAACCTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGGGGTTAGCTGTTTGT |
| Suggested primer 2: | GCAAAGACAAAGACCCAGCG |