Insertion junction: CLIP2.058586_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.058586
Chromosome:chromosome 11
Location:1837910
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre11.g467450 SOM3 S-adenosyl-L-methionine-dependent O-methyltransferase; (1 of 4) PTHR13600//PTHR13600:SF12 - LEUCINE CARBOXYL METHYLTRANSFERASE // LEUCINE CARBOXYL METHYLTRANSFERASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCACTAACCACCCATCCCCACCTACCACCCGCAATCCACTCCCCATCC
Internal bar code:TTCCTGTATTGCCTGTTATCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:68
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTAGTCACTGCGGCATT
Suggested primer 2:AAACCAACGAAAACGAGCGG