| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.058611 |
| Chromosome: | chromosome 3 |
| Location: | 3963437 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171250 | (1 of 1) IPR000104//IPR011989//IPR012677//IPR013083 - Antifreeze protein, type I // Armadillo-like helical // Nucleotide-binding alpha-beta plait domain // Zinc finger, RING/FYVE/PHD-type | 5'UTR | |
| Cre03.g171300 | GYX1 | (1 of 1) 1.1.3.15 - (S)-2-hydroxy-acid oxidase / Hydroxy-acid oxidase B; Glycolate oxidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGCATATTTGCATCAATCTATCTAAATATCAAGCTCCAGTCGAGAC |
| Internal bar code: | GTTACTAGGCGTTCGTGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1308 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACTGAAGGCTCACAGAC |
| Suggested primer 2: | AGAGGAGAGCAGAAGGGGAG |