| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.058625 |
| Chromosome: | chromosome 2 |
| Location: | 6347230 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387750 | (1 of 100) IPR001810 - F-box domain | 5'UTR | |
| Cre09.g387800 | FER1 | Pre-apoferritin; (1 of 2) K00522 - ferritin heavy chain (FTH1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGATGCACACCCGGCGCCACCAACTAGTCAGTTGCACGCTTCGCACCTG |
| Internal bar code: | GTGTGTGCTCGTGCTTACGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3603 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGCAGTTATCAGGCTTT |
| Suggested primer 2: | GCTCAGCATGTGATTCTGCG |