Insertion junction: CLIP2.058637_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.058637
Chromosome:chromosome 12
Location:2350079
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g508000 TIC40 (1 of 5) IPR006636 - Heat shock chaperonin-binding; Putative chloroplast enveloppe protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCGAAATACACGAATAGTCGCAATGGATGATAGAGAAGGAGGCAGATG
Internal bar code:AGTAACTAATGGAACAATTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:4692
LEAP-Seq percent confirming:65.7143
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:GATTCGCAGACCCCAACAGA
Suggested primer 2:GTATCGCACAACGCATGGTC