| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058649 |
| Chromosome: | chromosome 1 |
| Location: | 2101598 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011750 | (1 of 1) PTHR31353:SF1 - PROTEIN TAG-322 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGAGCGAGCGCCGCGTGTCGCGAACTGCGAGGTGGCTGCATGTTGGCT |
| Internal bar code: | ACAACATTGCGGGTGAGGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 55.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGCTGAATTCCTGGCGT |
| Suggested primer 2: | AAGCAGCAAGGAGACAGGAC |