| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.058658 |
| Chromosome: | chromosome 6 |
| Location: | 374568 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251500 | SPS1 | (1 of 1) K18787 - thermospermine synthase (ACL5); Spermine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCGCGCGTCGTCGTTGATGAGCGTGAGGCGCGGGTCGGCGAAGGCCT |
| Internal bar code: | TTACAGGGGTGGGATGTCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACCCATGGGCACTTGTT |
| Suggested primer 2: | TTGGCTTCCACTTGGGGTTT |