| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.058661 |
| Chromosome: | chromosome 13 |
| Location: | 2816842 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801539 | (1 of 1) PTHR14140:SF27 - PROTEIN SHISA-5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGGCAGTGGTGTAGGTTCAGGGCTCCCCTGCATAGGCTCGTGTCAGG |
| Internal bar code: | TATGAGGCCGGTGGAGGGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGAATGGTTTCTCTGCCT |
| Suggested primer 2: | CCAAGCATTTTGGCAGTCCC |