| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.058685 |
| Chromosome: | chromosome 6 |
| Location: | 578790 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g253250 | MADS2 | (1 of 1) PTHR11945:SF221 - SERUM RESPONSE FACTOR | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGGTTCCCCTCGCAAGGGAAAAGTGGGCTGAGCCCACGCCACGTCTT |
| Internal bar code: | TCTTAGGGGTACTGTCACTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGCCATATCTGGGGACT |
| Suggested primer 2: | CACATACCACCCCTCTGCTG |