Insertion junction: CLIP2.058700_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.058700
Chromosome:chromosome 13
Location:1410170
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g571950 (1 of 1) 2.1.1.104 - Caffeoyl-CoA O-methyltransferase / Trans-caffeoyl-CoA 3-O-methyltransferase 3'UTR
Cre13.g572000 TPM1,UPM2,SUMT2,COBA Putative tetrapyrrole methylase; (1 of 1) 2.1.1.198 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTGCTGCCGCTTTTACCAGCCAGCTCCATTGTCTCCAATCTCCATCGC
Internal bar code:CGCTGGCCACGATTAGCAACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3543
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAGAGGTGAGCTCCAGGG
Suggested primer 2:GGGTAGCATCTAGTGGACGC