| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.058701 |
| Chromosome: | chromosome 15 |
| Location: | 1246881 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638400 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATCGGACGTTGTGCGACAGAAGTTTAGATAAAAAGATAATGCCTGTAT |
| Internal bar code: | CCTTAACTCCGACGCAATCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3290 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTTTGCCAGCTGCTTGG |
| Suggested primer 2: | AAGCAAGATTCGAGGGGGTG |