| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058710 |
| Chromosome: | chromosome 7 |
| Location: | 2053941 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325850 | MAW8 | Membrane-associated hydroxyproline-rich glycoprotein 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCCTGTCACTACTCGCCCACCAGCAGCAGCAAGTCAGCAATGTCTCG |
| Internal bar code: | GGTTGTACATTGCTCACAGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTACAACGTGCAGATGCT |
| Suggested primer 2: | GCTGGCAGGCTGGTAGTAAA |