| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.058714 |
| Chromosome: | chromosome 10 |
| Location: | 5208656 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455900 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAACTTGAAACAACTCATAGAGCGTGGTCGCCTTGCACACGCAACAT |
| Internal bar code: | TTCTACATCTGTGCCGATCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCTGGTCCTTGAGTAGT |
| Suggested primer 2: | CACACGAACACCTGCACAAG |