Insertion junction: CLIP2.058716_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.058716
Chromosome:chromosome 5
Location:713281
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g245350 (1 of 1) IPR003582//IPR009011 - ShKT domain // Mannose-6-phosphate receptor binding domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGGTATAGGTGTCCGGTTTGGTGACGACCAAGGTGACGACCTGTTAGT
Internal bar code:GTTGTCGGCCGATTTAAAACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3619
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:89
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:TATTATCGACCACCGCTGCC
Suggested primer 2:GGAGGGAAAGGCGGATGAAA