Insertion junction: CLIP2.058720_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.058720
Chromosome:chromosome 5
Location:2325904
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre05.g238280 FAP205 Flagellar Associated Protein 205 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTGGTCCGGAAACCGGCACACGTGGGTTTGGAGCCCTGCCTGCACACA
Internal bar code:GTCGTCGTGTTGGTAGAGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:541
LEAP-Seq percent confirming:7.69231
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GACGCACCACCTGAAGAAGA
Suggested primer 2:CGTCACGGGTTCCCTATAGC