| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.058724 |
| Chromosome: | chromosome 5 |
| Location: | 3386544 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232900 | (1 of 4) PF02181 - Formin Homology 2 Domain (FH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGAGGGGGGAGAGAGGGAGGAGAGGATTAGGGCATCCACGCAAGGC |
| Internal bar code: | GGTGGTGAGATGTGTATAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 67.8571 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGATCACCTCCAACCGC |
| Suggested primer 2: | AACCCCTATCCTCCACCTCC |