| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.058726 |
| Chromosome: | chromosome 16 |
| Location: | 5571206 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681600 | CSV14 | Chlamydomonas-specific family protein | 3'UTR |
| Cre16.g681700 | NUOS4B | (1 of 2) K03937 - NADH dehydrogenase (ubiquinone) Fe-S protein 4 (NDUFS4); NADH:ubiquinone oxidoreductase subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTCAGTACTGACGCCGTCGTCCCTGGCTACGCTCTATCTTTGCGTGG |
| Internal bar code: | GGGGGGTGGAAAGAAATTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5119 |
| LEAP-Seq percent confirming: | 47.619 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTGAACCACGCCCAAAG |
| Suggested primer 2: | CGGCGCTAGGATCCAGAAAT |