| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.058751 |
| Chromosome: | chromosome 8 |
| Location: | 3792700 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTACTTGATGCTGGATTGCTAGCGCAAGCAAGCACTCAGTGCGATAT |
| Internal bar code: | CATAAGACCGGGACAATGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1711 |
| LEAP-Seq percent confirming: | 93.5484 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACACGTCGTGTCACTGA |
| Suggested primer 2: | TGCCCTCGCCATTTGAAGAT |