| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058757 |
| Chromosome: | chromosome 12 |
| Location: | 9089660 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544401 | (1 of 1) PTHR20934:SF0 - TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAATTGGGTCGGTACAATCACACACTAGCGAATGAGGCTTGGAGACGT |
| Internal bar code: | ACCTTAAATTAAGTCGTGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACCTCCATCTCATCCGC |
| Suggested primer 2: | GAGGACAGTTCTGGAAGGCC |