| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058765 |
| Chromosome: | chromosome 4 |
| Location: | 3054838 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225250 | (1 of 3) PF14494 - Domain of unknown function (DUF4436) (DUF4436) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAACAACTTCGCTGTCTGTGTGCTTGCACTCTCAGATTGCCGCCTCT |
| Internal bar code: | ATGTTTAATTGGATCATGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1784 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGTCCTGTACGGCAGT |
| Suggested primer 2: | GCCGCAAGATTTAGACGCTG |