| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.058765 |
| Chromosome: | chromosome 4 |
| Location: | 3054842 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225250 | (1 of 3) PF14494 - Domain of unknown function (DUF4436) (DUF4436) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAGAGGAAAACCCGACCGGCGCCGAAGGGTTAGGCACTCCGGCAAATC |
| Internal bar code: | ATGTTTAATTGGATCATGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2043 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGCAAGATTTAGACGCTG |
| Suggested primer 2: | CTTGTGTCCTGTACGGCAGT |