Insertion junction: CLIP2.058768_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.058768
Chromosome:chromosome 7
Location:4664476
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g344800 TNP21,CSB33 (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACGTTCCGGCTCCACCACTAACTTTCGCATATTACTCTACATTTCCCT
Internal bar code:GGATTGATATGCTGAAGAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1513
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGGCTGGGTTGATAACG
Suggested primer 2:CCAACTTTTGGCAGCAGGTC