| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058769 |
| Chromosome: | chromosome 16 |
| Location: | 3019769 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664000 | HLM25 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase; Histone-lysine N-methyltransferase | 3'UTR |
| Cre16.g664050 | (1 of 1) PF02893//PF16016 - GRAM domain (GRAM) // Domain of unknown function (DUF4782) (DUF4782) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACGCATCTTTTAGCGCCGTTCACTAAGAGCGTGCCACACTTGCAGAGC |
| Internal bar code: | CGGGGGCACGTATGGTCTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2535 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAACGTGGACGGGTAAACC |
| Suggested primer 2: | AGTACAGAACGAGCGTGCAA |