| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058770 |
| Chromosome: | chromosome 7 |
| Location: | 3704281 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337750 | EBM5 | (1 of 3) PTHR31451//PTHR31451:SF1 - FAMILY NOT NAMED // MANNAN ENDO-1,4-BETA-MANNOSIDASE 2-RELATED; Mannan endo-1%252C4-beta-mannosidase | 3'UTR |
| Cre07.g337800 | MRPS17,uS17m | (1 of 3) PTHR10744 - 40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER; Mitochondrial ribosomal protein S17 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTGTCTATGAATGACATTCATGGGTGCAATAATGGCACCCCGATTGC |
| Internal bar code: | GAGCTGACTTTCGTCATGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2726 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCGTCTGCAGGAAGTTT |
| Suggested primer 2: | AGTGTAGTGAGTTGGGTGCG |