| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.058778 |
| Chromosome: | chromosome 16 |
| Location: | 1041617 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649250 | (1 of 4) PF13911 - AhpC/TSA antioxidant enzyme (AhpC-TSA_2) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGCGTGTTGAAGGGAGCCTGTTTATACGTGTGTGCCTGTATGCGTCT |
| Internal bar code: | ATCGGGATCAGCATGTTGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3342 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTAAAGCGCGGGGATCA |
| Suggested primer 2: | CGTTACATAGCGCTGCAACC |