| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058800 |
| Chromosome: | chromosome 13 |
| Location: | 3725834 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588495 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCAGAATTTTTGACAACACTTACACATCATAGCCGCGCCCAACACA |
| Internal bar code: | GTACAATGCCCCGTTGTCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2476 |
| LEAP-Seq percent confirming: | 96.7213 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGAGTTGCCCCAAGGT |
| Suggested primer 2: | GTTTCGTGGGTGGTTTCGTG |