| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058801 |
| Chromosome: | chromosome 6 |
| Location: | 146414 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250100 | HSP70B | Heat shock protein 70B; (1 of 1) PTHR19375//PTHR19375:SF147 - HEAT SHOCK PROTEIN 70KDA // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGGTAGCCTGCATGGGGGTTCTAGGTTGTTCCATGCCGAGGCCGTC |
| Internal bar code: | AGCGGCGTCGCTATGACGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCACCACCACCATCCTCC |
| Suggested primer 2: | TGACACCACGAACGTACCTG |