| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058805 |
| Chromosome: | chromosome 2 |
| Location: | 3533566 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099150 | SNAPA1,FAP375 | Alpha-SNAP, SNARE complex protein; (1 of 1) K15296 - alpha-soluble NSF attachment protein (NAPA, SNAPA, SEC17) | 3'UTR |
| Cre02.g099200 | (1 of 1) K06100 - symplekin (SYMPK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGATTCTGAGTGCGACAACGGGGTCAGGGGATTCCGACAGTGGCTTAA |
| Internal bar code: | CTTCATTTACTTTGCAGAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1609 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGCATGCATGTGTACGG |
| Suggested primer 2: | GCGTGGGGTATTGGGTTGTA |