Insertion junction: CLIP2.058812_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.058812
Chromosome:chromosome 16
Location:5389909
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683100 (1 of 239) IPR016024 - Armadillo-type fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAACATCAAAGCGCGGCCACATCATCCCGATGCATGGCAATGTTGGAC
Internal bar code:TCGTTAGTTGAGTCTCAAGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:751
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGTCATGGGTTCGGGTA
Suggested primer 2:ATAGCTCCACGCGTGAACAA