| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.058816 |
| Chromosome: | chromosome 2 |
| Location: | 3905927 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101750 | (1 of 1) PF04484 - Family of unknown function (DUF566) (DUF566) | 3'UTR | |
| lncRNA_TCONS_00040754 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTCGACCGAAGGGCATTGTCAACTGTGTGTCCAGGATTCCAGGTCCG |
| Internal bar code: | ACCAGCTAATTCATCAAAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5590 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGTTCGGACTTGACCAG |
| Suggested primer 2: | GTACCCCGTAGCACGATCTG |