| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058835 |
| Chromosome: | chromosome 7 |
| Location: | 4405533 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343101 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCGCACAATACCCAGACGTCAAATTCTTTGGTGGGTCTCTTTGGGG |
| Internal bar code: | TAAACTTTGGGCCATGTCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1932 |
| LEAP-Seq percent confirming: | 67.2727 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGGGAGCCATTGTTTCT |
| Suggested primer 2: | CTGCTGCCGCCATTTCTTTT |