Insertion junction: CLIP2.058843_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.058843
Chromosome:chromosome 17
Location:3467152
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g723750 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTACCCGTCACATCTCACGAGCCAGGGCTGCCTTGCTTATCTCAAGCT
Internal bar code:TATAATTACTGGGGCGCGGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:741
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CATATGCCACGCAGGTTTCG
Suggested primer 2:AAACGCCGTGACTTCTGCTA