| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.058850 |
| Chromosome: | chromosome 3 |
| Location: | 2590648 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160200 | P4H12,PHX7,PFH12,Cr-P4H-1 | (1 of 2) IPR003582//IPR005123//IPR006620//IPR027450 - ShKT domain // Oxoglutarate/iron-dependent dioxygenase // Prolyl 4-hydroxylase, alpha subunit // Alpha-ketoglutarate-dependent dioxygenase AlkB-like; Prolyl 4-hydroxylase 12 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCGTTAAATCGCAGTGCTGTTTGTATTATGAATACGTGAATGCGCGG |
| Internal bar code: | ATTCCTTGCCCTGGTGTAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3075 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACCTCCTGTCCTCCTCA |
| Suggested primer 2: | GTAGTCGCACTCCTCATCCG |