| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058858 |
| Chromosome: | chromosome 1 |
| Location: | 3659095 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023750 | CDS | ||
| Cre01.g023773 | (1 of 6) IPR000253//IPR008984 - Forkhead-associated (FHA) domain // SMAD/FHA domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTTCTCGTCCTCCCAGCGACGTATTCTATTCTTCTCCTCGTCCTTGA |
| Internal bar code: | GTACGTGCTACGACGGGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1279 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATCCGCACGTCGCTACTC |
| Suggested primer 2: | AAGACTGATGGGCATTGGGG |