| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058880 |
| Chromosome: | chromosome 12 |
| Location: | 6544880 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538150 | (1 of 1) K05019 - chloride channel, nucleotide-sensitive, 1A (CLNS1A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTGCTCTCGTGCTGGAAAGACGGTGGCGGGGGCATGCCACCGGTTG |
| Internal bar code: | TTGATGTCAGTGCTTTAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 23.5294 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGAGGACACAAGTTGAGA |
| Suggested primer 2: | GCACAGCAGTTATGACGCAG |