| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058882 |
| Chromosome: | chromosome 15 |
| Location: | 1437372 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641650 | HLM22 | (1 of 4) PTHR10887:SF331 - HELICASE MOV10L1-RELATED; Histone-lysine N-methyltransferase | 3'UTR |
| lncRNA_TCONS_00236652 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGGTAGTTCAGTCCCCTTTCAGGGCTGGTATCGGGACGCTGTTGCA |
| Internal bar code: | GCTTAGGACTTATAAGGTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1867 |
| LEAP-Seq percent confirming: | 27.2727 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACTCGGAGCTTTCGAA |
| Suggested primer 2: | ACGGTGGTTACGGATCCAAC |