| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.058900 |
| Chromosome: | chromosome 14 |
| Location: | 2726287 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625950 | PAP4 | (1 of 1) 2.7.7.19//2.7.7.72 - Polynucleotide adenylyltransferase / RNA adenylating enzyme // CCA tRNA nucleotidyltransferase / tRNA-nucleotidyltransferase; Class-II RNA nucleotidyl transferase 4, mitochondrial | 3'UTR |
| Cre14.g626000 | (1 of 6) PF03547 - Membrane transport protein (Mem_trans) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGGTGCATGCGCGGTAATAGCAGGATACGGTGTCACTGTGAAGGCCT |
| Internal bar code: | TAGTTGGGGTAATTAAAGCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCAACAGCAACAGCAG |
| Suggested primer 2: | CCTACGGGACTTCACCAACC |