| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.058903 |
| Chromosome: | chromosome 13 |
| Location: | 3008855 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584135 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGCGCGAGCGCACGCAAGCTCGACGGGGTTGACGCCTGGCTGCGGCG |
| Internal bar code: | AACGCCAGTCCTGTGGCCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4651 |
| LEAP-Seq percent confirming: | 92.233 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCACACACACACACACAC |
| Suggested primer 2: | TGTGACTACGGTTTCCACGG |