| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058915 |
| Chromosome: | chromosome 3 |
| Location: | 3494805 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167250 | (1 of 1) PF05673 - Protein of unknown function (DUF815) (DUF815) | 3'UTR | |
| Cre03.g167300 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTGGCCACTACTCTCGCCCGTCGACGACCGCCGCTGGATGCGACCGT |
| Internal bar code: | TTGAAAGCCCAACATTATACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3801 |
| LEAP-Seq percent confirming: | 98.9362 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCGGATACTGTCGAGAG |
| Suggested primer 2: | CGAGTCCTGGCCTTTCATGT |