| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058929 |
| Chromosome: | chromosome 8 |
| Location: | 577249 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359800 | 5'UTR | ||
| Cre08.g359850 | FBB11 | Flagellar and Basal Body Protein 11; (1 of 2) PF13864 - Calmodulin-binding (Enkurin) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGAGGCAGGTATACCGCATCAATTACGTATAGTGTATTCATACCGTGC |
| Internal bar code: | TGCGGGTTCCGTGGTTCGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3178 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCAATGAAGTGCAGCAG |
| Suggested primer 2: | TGAGTGACACGGAAGGCTTC |