Insertion junction: CLIP2.058952_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.058952
Chromosome:chromosome 2
Location:8165533
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g144006 (1 of 17) PF13833 - EF-hand domain pair (EF-hand_8) CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGATTGTGATCCTGTCCGACGGGCGCACGCTGGAGCCCAACGGCGGCG
Internal bar code:GAGATTTTTCTTAGGAGGTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:99
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGGATGAGGGCATGTGT
Suggested primer 2:CAGTAGCAGTCTCGGTAGCG