Insertion junction: CLIP2.058959_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.058959
Chromosome:chromosome 6
Location:3900301
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278189 (1 of 11) PF00999 - Sodium/hydrogen exchanger family (Na_H_Exchanger) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCACCAACACCCATGAGAGACGCCCCTCTGAGGCACGCCACGCCACGC
Internal bar code:TAGTTGCCGTATGACCGGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:400
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTCCCCTTCAGGTGTTC
Suggested primer 2:GCTTGGCAGCATGGTTTTGA