| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.058961 |
| Chromosome: | chromosome 14 |
| Location: | 2360669 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623125 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGCAACCGCGCCTTTCACCCCGGCAATCAGCCCGGCACACCGTGCCGG |
| Internal bar code: | GCTGCTGCCGTAGGAGCAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGTGCTGCGTGTTTGTA |
| Suggested primer 2: | CGCCACAGTTAACGACAAGC |